Cell Lines and Primary Tissues for InVitro Evaluation ofVaccine Efficacy 153

Anthony Meager

6.1 Introduction 153

6.2 Measurement of Antigen Expression 155

6.3 Post-Vaccination Testing 158

6.3.1 Ex-Vivo Detection of Antigen-Specific T Cells 160

6.3.1.1 ELISPOT Assay 160

6.3.1.2 Cytokine Capture Assay and Intracellular Cytokine Staining 162

6.3.1.3 Measurement of T-Cell Cytotoxicity 163

6.3.2 Current Knowledge on T-Cell Responses in Vaccine Trials 165

6.4 Future Directions 167 References 168

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