Edge Effect

Edge effects are another frequent phenomena in cellular screens, and involve a number of different factors. Thermal gradients across the plates have been described as one main reason [34], as this leads to irregular patterns ofcell adhesion and distribution in the well. Coating of the plates to improve cell adhesion, adding a pH stabilizer such as HEPES, and not using the peripheral wells can reduce the problem. Adjusting the media volume may also help, with a depth of not more than 2-5 mm being recommended for good gaseous diffusion [10, 35]. Incubation of the newly seeded plates for 1-2 h at room temperature minimizes the thermal gradient during cell settlement and results in more even cell distribution and adhesion, as shown for CHO cells [34] and (by the present authors) for BHK cells.

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