In general, in order to be cost-effective, an HTS screen must provide a robust and reproducible signal and an adequate throughput ofabout 20 to 100 000 samples per day. For cell-based assays, both the cell line itself and the cell production process must be selected to optimize stability and the signal window. The ideal cell for HTS is fast growing, has good adherence, a stable cellular phenotype, a broad tolerance against solvents, and is insensitive against culture modifications. All of these parameters must to be tested for each cell line and each assay, using HTS equipment and conditions early during cell development (see Fig. 5.1).
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