Analysis of at least two blood cultures (aerobic, anaerobic) at the same time, i.e., 2 x10 ml of venous blood, is mandatory. Since bacteremia may be low-grade (<10 microorganisms/ml), multiple blood cultures may be required, in particular in case of negative results (> 50 % in cases of severe sepsis!) of the initial hemocultures. They should best be taken during the rise in body temperature, i.e., just before the fever spike. When antimicrobial therapy has already been started, blood should be drawn before repeated antibiotic administration.

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