Assessment of Thiamin Status

Thiamin status can be assessed using methods that measure thiamin or its metabolites in plasma, ery-throcytes and urine (Table 2). Samples are acidified to stabilize the thiamin and precipitate any protein. Usually, thiamin is oxidized to thiochrome (Figure 2) using cyanogen bromide in alkaline solution and measured by fluorescence with or without chroma-tography. Concentrations of thiamin in urine and plasma tend to reflect dietary intake, being high when intake is adequate and low when dietary sources are poor. Erythrocyte thiamin is mainly in the form of the coenzyme TDP, which can be extracted from washed erythrocytes, derivatized as described previously, and quantified by highperformance liquid chromatography. The most

Table 2 Biochemical assessment of thiamin status

Test Acceptable Marginal risk High risk

Table 2 Biochemical assessment of thiamin status

Test Acceptable Marginal risk High risk

Urinary thiamin

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