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3. Lysine content of the mixture (mg per g protein):

Food mg lys per g protein g component per

100 g mixture

3. Lysine content of the mixture (mg per g protein):

Food mg lys per g protein g component per

100 g mixture

Chicken (l590/19.2) x 20

Weighted mean = (364 + 734 +1656)/(10 +10 + 20) = 60 mg lys per g protein

To calculate the amino acid content of a combination of food proteins, as in a processed food based on several protein sources or in a mixed diet, a weighted mean of the published or analytical results of each component should be used, as illustrated in Table 2.

Amino Acid Scoring Pattern

For infants younger than 1 year, the scoring pattern should be based on the amino acid composition of breast milk, even if some EAAs in human milk exceed minimum requirements for infants of this age. For example, infants consuming cow's milk proteins, which have less sulfur-containing amino acids than human milk, show adequate growth and nitrogen balance. Thus, although the use of a scoring pattern based on human milk composition may somewhat underestimate the protein quality of some foods for infants, there is consensus to accept errors on the side of safety for this highly vulnerable age group.

International expert committees (WHO, FAO, UNU, and CCVP) have agreed that the scoring pattern proposed in the 1980s for preschool children—based on studies of amino acid requirements at the Institute of Nutrition of Central America and Panama and on recommendations of protein intake by FAO, WHO, and UNU—is robust and represents the best available estimate of EAA requirements for this age group. Published nitrogen balance studies on older children and adults have experimental flaws, and only a limited

Table 3 Amino acid scoring patterns for infants under 1 year and for older children and adults (mg amino acid per g protein)3

Amino acid Infant Older children Egg, cow's milk,

Table 3 Amino acid scoring patterns for infants under 1 year and for older children and adults (mg amino acid per g protein)3

Amino acid Infant Older children Egg, cow's milk,

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