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distribution of LDL subfractions; however, age, gender, and environmental factors strongly influence the penetrance. HDL subfractions have been resolved using a similar technique, with a polyacry-lamide gradient ranging from 4 to 30%, into five subclasses (HDL3c, HDL3b, HDL3a, HDL2a, and HDL2b). More recently 11-14 subclasses have been described, including ^-migrating particles, using an improved electrophoresis technique. The clinical importance of these subfractions is still under investigation.

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