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or interactions)

production of enzymes favoring the invasion/translocation through the epithelium, etc.), with the presence of active substances in the probiotic or product (immunoactive substances, toxic compounds, etc.), or with antibiotic resistance. It is clear that strains harboring transferable antibiotic resistance genes should not be used. In this context the specific risks related to each probiotic strain must be carefully identified.

Guidelines are needed to test the safety of probio-tics. However, taking into account the great diversity of probiotic microorganisms, it is necessary to identify the specific risks associated with the respective strains, as well as the risk factors associated with the host and the possible interactions between probiotic-host-food components in order to assess the safety of these products. Additional epidemiolo-gical surveillance and follow-up of novel strains should be conducted. In this context, the specific risks related to each probiotic strain must be carefully identified. With regard to this, knowledge of mechanisms involved is a key factor not only for the assessment of health effects but also for the safety aspects of probiotics.

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