Measurement of Choline and Choline Esters

Radioisotopic, high-pressure liquid chromatogra-phy, and gas chromatography/isotope dilution mass spectrometry (GC/IDMS) methods are available for measurement of choline. However, these existing methods are cumbersome and time consuming, and none measures all of the compounds of choline derivatives. Recently, a new method has been established for quantifying choline, betaine, acetyl-choline, glycerophosphocholine, cytidine diphospho-choline, phosphocholine, phosphatidylcholine, and sphingomyelin in liver, plasma, various foods, and brain using liquid chromatography/electrospray ionization-isotope dilution mass spectrometry (LC/ ESI-IDMS).

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